recombinant il 2 (R&D Systems)
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R&D Systems
recombinant il 2
Recombinant Il 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+il+2/Recombinant+Human+IL-2+Protein/pm42026075-200-17-19
Average 96 stars, based on 903 article reviews
Recombinant Il 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+il+2/Recombinant+Human+IL-2+Protein/pm42026075-200-17-19
Average 96 stars, based on 903 article reviews
recombinant il 2 - by Bioz Stars,
2026-09
96/100 stars
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Recombinant:Article Title: Human umbilical cord mesenchymal stromal cells therapy for neuromyelitis optica spectrum disorder: a phase 1/2a trial. Article Snippet: Xiao-Ying Yao, Zhong-Jiao Lu, Nan Zhao, Li Xie, Chun-Ran Xue, Yi-Shu Wang, Xu-Zhong Pei, Meng-Ze Zhang, Ying Zhang, Wen-Bin Wan, Yan Lin, Ye Deng, Xi-Ying Wang, Yi Chen, He Zhu , Lin-Feng Li, Hui-Ming Xu, Jie Ding, Yi-Fan Wu, Ze Wang, Ya-Ying Song, Kan Wang, Hui-Ying Qiu, Yan Zhou, Yu-Yan Jin, Li-Ping Ni, Jia-Li Yan, Quan Guo, Jia-Hui Xue, Yu-Fang Mei, Bi-Yun Qian3,6, Hao-Jun Yu 1,7,8✉ and Yang-Tai Guan 2✉ Article Title: Multiplexed high-content imaging uncovers morphological diversity of lymphocyte activation and dysfunction Article Snippet: Isolated T cells were cultured in round-bottom 96-well plates or flat-bottom 24-well plates at 1×10 6 cells/mL in StemPro TM 34-SFM (Gibco TM , #10639011) supplemented with 10% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin. .. Unless specified, Article Title: O-GlcNAcylation and low glycolysis underpin Th2 polarization by dendritic cells Article Snippet: For Th1/2 polarization, 5.000 DCs that were stimulated for 24h were co-cultured with 20.000 allogenic naïve CD4 T cells in the presence of 20 pg/mL Staphylococcal Enterotoxin B (SEB; #S4881, Sigma) in a cell culture treated flat-bottom 96 well plate. .. After 5-7 days, cells were transferred to a flat-bottom 24 well plate well containing 1 mL of media that was supplemented with 42 IU/mL Article Title: Materials and methods relating to immunogenic epitopes from human papilloma virus Article Snippet: Transfection of APCs were done using the Lonza 4D Nucleofector, primary P3 buffer, program E0117 (Lonza, MD, USA) and incubated in BCM-10% human serum, IL-4 without any Anti-Anti. .. Twenty four hours later, on day 1, APCs were washed and incubated with thawed whole HPV+ HNSCC PBMCs at a ratio of 1:2 (200,000 APCs: 400,000 PBMCs) in a 24-well plate in BCM supplemented with 20 U/mL Article Title: Modeling HIV infection, treatment, rebound, and intervention in human immune organoids Article Snippet: PBMCs were thawed, and NK cells were isolated by negative selection using NK-negative selection kits (Miltenyi Biotec, #130-092-657). .. NK cells were cultured overnight in R10 medium (RPMI 1640, with 10% FBS, 1% L-glutamine, 1% sodium pyruvate, and 1% PSA Penicillin/Streptomycin/ Actinomycin) supplemented with 300 IU/ml Article Title: Loss of Vpr-driven TRAIL-R2 expression protects HIV-infected cells from non-canonical NK cell TRAIL attack Article Snippet: Prior to isolating CD4 + T cells, a non-TC treated sterile 24-well plate (Corning, Inc., Cat.#CLS3738) was coated with 400μL per well of 2μg/mL anti-CD3 antibody (Biolegend, Cat.#317326), diluted in Coating Buffer for at least 2hrs at 37°C, or overnight at 4°C. .. Before plating cells, each well was washed twice with sterile PBS, then given 0.4mL R10 media containing 10ng/mL Article Title: Selective Immune Silencing by Targeted TGF-β Agonists Article Snippet: .. For human in vitro cultures, T cells were plated at 0.75 × 10 6 cells/mL in flat-bottom 12-well plates coated overnight with 5 μg/mL InVivoMAb anti-human CD3 (BE0001, Bio X Cell) and 2.5 μg/mL InVivoMAb anti-human CD28 (BE0248, Bio X Cell) in the presence of 10 ng/mL Article Title: Materials and methods relating to immunogenic epitopes from human papilloma virus Article Snippet: APCs were either peptide pulsed with HPV16-epitopes, or transfected with whole HPV-antigen encoded in mammalian expression plasmid pCDNA3.2 (Invitrogen, CA, USA). .. APCs were washed and incubated with thawed whole HPV+ HNSCC PBMCs at a ratio of 1:2 (200,000 APCs: 400,000 PBMCs) supplemented with 20 U/mL Activation Assay:Article Title: Human umbilical cord mesenchymal stromal cells therapy for neuromyelitis optica spectrum disorder: a phase 1/2a trial. Article Snippet: Xiao-Ying Yao, Zhong-Jiao Lu, Nan Zhao, Li Xie, Chun-Ran Xue, Yi-Shu Wang, Xu-Zhong Pei, Meng-Ze Zhang, Ying Zhang, Wen-Bin Wan, Yan Lin, Ye Deng, Xi-Ying Wang, Yi Chen, He Zhu , Lin-Feng Li, Hui-Ming Xu, Jie Ding, Yi-Fan Wu, Ze Wang, Ya-Ying Song, Kan Wang, Hui-Ying Qiu, Yan Zhou, Yu-Yan Jin, Li-Ping Ni, Jia-Li Yan, Quan Guo, Jia-Hui Xue, Yu-Fang Mei, Bi-Yun Qian3,6, Hao-Jun Yu 1,7,8✉ and Yang-Tai Guan 2✉ Incubation:Article Title: Materials and methods relating to immunogenic epitopes from human papilloma virus Article Snippet: Transfection of APCs were done using the Lonza 4D Nucleofector, primary P3 buffer, program E0117 (Lonza, MD, USA) and incubated in BCM-10% human serum, IL-4 without any Anti-Anti. .. Twenty four hours later, on day 1, APCs were washed and incubated with thawed whole HPV+ HNSCC PBMCs at a ratio of 1:2 (200,000 APCs: 400,000 PBMCs) in a 24-well plate in BCM supplemented with 20 U/mL Article Title: Materials and methods relating to immunogenic epitopes from human papilloma virus Article Snippet: APCs were either peptide pulsed with HPV16-epitopes, or transfected with whole HPV-antigen encoded in mammalian expression plasmid pCDNA3.2 (Invitrogen, CA, USA). .. APCs were washed and incubated with thawed whole HPV+ HNSCC PBMCs at a ratio of 1:2 (200,000 APCs: 400,000 PBMCs) supplemented with 20 U/mL Cell Culture:Article Title: Modeling HIV infection, treatment, rebound, and intervention in human immune organoids Article Snippet: PBMCs were thawed, and NK cells were isolated by negative selection using NK-negative selection kits (Miltenyi Biotec, #130-092-657). .. NK cells were cultured overnight in R10 medium (RPMI 1640, with 10% FBS, 1% L-glutamine, 1% sodium pyruvate, and 1% PSA Penicillin/Streptomycin/ Actinomycin) supplemented with 300 IU/ml Sterility:Article Title: Loss of Vpr-driven TRAIL-R2 expression protects HIV-infected cells from non-canonical NK cell TRAIL attack Article Snippet: Prior to isolating CD4 + T cells, a non-TC treated sterile 24-well plate (Corning, Inc., Cat.#CLS3738) was coated with 400μL per well of 2μg/mL anti-CD3 antibody (Biolegend, Cat.#317326), diluted in Coating Buffer for at least 2hrs at 37°C, or overnight at 4°C. .. Before plating cells, each well was washed twice with sterile PBS, then given 0.4mL R10 media containing 10ng/mL In Vitro:Article Title: Selective Immune Silencing by Targeted TGF-β Agonists Article Snippet: .. For human in vitro cultures, T cells were plated at 0.75 × 10 6 cells/mL in flat-bottom 12-well plates coated overnight with 5 μg/mL InVivoMAb anti-human CD3 (BE0001, Bio X Cell) and 2.5 μg/mL InVivoMAb anti-human CD28 (BE0248, Bio X Cell) in the presence of 10 ng/mL |
